Protein List


Summary

Result Statistics

Figure 1: Protein profile heatmap (Cell colour represents the log2(ratio) to the average area across different samples)



Figure 2. The volcano plot for proteins.

(a)

Figure 3. The distribution of feature vector ratio: (a) By quality. (b) By intensity.

(a)
(b)

Figure 4. (a) RT shift distribution; (b) M/Z shift distribution.

(a)
(b)
Table 1. Result filtration parameters.
Retention time≥0
Retention time≤150
Feature significance≥0
Feature fold change≥1
Quality≥0
Avg. Area≥0E0
Charge≥1
Charge≤10
Confident sample number≥7
With peptide IDtrue
Protein significance≥13
Protein fold change≥2
Significance methodPEAKSQ
Confident unique supports≥2
NormalizationUse internal standard proteins
Table 2. Statistics of filtered result.
Features125349
Features with ID65760
Feature vectors12260
Feature vectors with ID12260
Protein groups55
Table 3. Search Parameters
Quantification type: Label free quantification
Mass Error Tolerance: 30.0 ppm
Retention Time Shift Tolerance: 3.0 min
Dependent on PID: 35,42,50,56,90,133,112,277,115,281,118,285,12
1,289,124,293,171,297,174,301,177,305,161,309,165,313,168,317
,201,321,204,325,207,329,210,333,236,337,239,341,244,345,255,
349,263,353,271,357
FDR Threshold: 1%
Samples: 12 samples in 2 groups
: MS3GM MS7GM MS14GM MS17GF
: MS18GF MS20GF LS1GM LS4GM
: LS10GM LS6GF LS8GF LS12GF
Reference Sample: LS6GF
Training Samples: LS1GM, LS10GM (auto detected)