Protein List


Summary

Result Statistics

Figure 1: Protein profile heatmap (Cell colour represents the log2(ratio) to the average area across different samples)



Figure 2. The volcano plot for proteins.

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Figure 3. The distribution of feature vector ratio: (a) By quality. (b) By intensity.

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Figure 4. (a) RT shift distribution; (b) M/Z shift distribution.

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Table 1. Result filtration parameters.
Retention time≥0
Retention time≤150
Feature significance≥0
Feature fold change≥1
Quality≥0
Avg. Area≥0E0
Charge≥1
Charge≤10
Confident sample number≥7
With peptide IDtrue
Protein significance≥13
Protein fold change≥2
Significance methodPEAKSQ
Confident unique supports≥2
NormalizationUse internal standard proteins
Table 2. Statistics of filtered result.
Features125584
Features with ID66723
Feature vectors12188
Feature vectors with ID12188
Protein groups81
Table 3. Search Parameters
Quantification type: Label free quantification
Mass Error Tolerance: 30.0 ppm
Retention Time Shift Tolerance: 3.0 min
Dependent on PID: 35,42,50,56,90,133,112,277,115,281,118,285,12
1,289,124,293,171,297,174,301,177,305,161,309,165,313,168,317
,201,321,204,325,207,329,210,333,236,337,239,341,244,345,255,
349,263,353,271,357
FDR Threshold: 1%
Samples: 12 samples in 2 groups
: MS3GM MS7GM MS14GM MS17GF
: MS18GF MS20GF AK7GM AK16GM
: AK22GM AK11GF AK12GF AK13GF
Reference Sample: AK11GF
Training Samples: AK16GM, AK12GF (auto detected)